SrasV1, Main, Exploration, bibRecord, 005A84

Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine

Identifieur interne : 005A84 ( Main/Exploration ); précédent : 005A83; suivant : 005A85

Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine

Auteurs : HAI PANG [République populaire de Chine] ; YINGGANG LIU [République populaire de Chine] ; XUEQING HAN [République populaire de Chine] ; YANHUI XU [République populaire de Chine] ; FUGUO JIANG [République populaire de Chine] ; DONGLAI WU [République populaire de Chine] ; XIANGANG KONG [République populaire de Chine] ; Mark Bartlam [République populaire de Chine] ; ZIHE RAO [République populaire de Chine]

Source :

Journal of general virology [ 0022-1317 ] ; 2004.

RBID : Pascal:04-0551737

Descripteurs français

Pascal (Inist)
- Coronavirus, Prévention, Immunité humorale, Grave, Malade état grave, Aigu, Voie respiratoire, Conception, Protéine, Syndrome respiratoire aigu sévère, Vaccin, Immunoprophylaxie, Microbiologie, Virologie, Forme grave.
Wicri :
- topic : Vaccin.

English descriptors

KwdEn :
- Acute, Coronavirus, Critically ill, Design, Humoral immunity, Immunoprophylaxis, Microbiology, Prevention, Protein, Respiratory tract, Severe, Severe acute respiratory syndrome, Vaccine, Virology.

Abstract

Some of the structural proteins of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for the induction of protective humoral responses and the development of an effective vaccine. Rabbit antisera were prepared using full-length N and M proteins and eight expressed fragments covering the S protein. Antisera to S and M proteins were found to have different neutralizing titres towards SARS-CoV infection in vivo, ranging from 1:35 to 1:128. Antiserum to the N protein did not contain neutralizing antibodies. Epitopes inducing protective humoral responses to virus infection were located mainly in the M protein and a region spanning residues 13-877 of the S protein. The neutralizing ability of antisera directed against the expressed structural proteins was greater than that of convalescent patient antisera, confirming that, as immunogens, the former induce strong, SARS-CoV-specific neutralizing antibody responses. The in vitro neutralization assay has important implications for the design of an effective, protein-based vaccine preventing SARS-CoV infection.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000786
to stream PascalFrancis, to step Curation: 000204
to stream PascalFrancis, to step Checkpoint: 000791
to stream Main, to step Merge: 005F42
to stream Main, to step Curation: 005A84

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term>
<term>Coronavirus</term>
<term>Critically ill</term>
<term>Design</term>
<term>Humoral immunity</term>
<term>Immunoprophylaxis</term>
<term>Microbiology</term>
<term>Prevention</term>
<term>Protein</term>
<term>Respiratory tract</term>
<term>Severe</term>
<term>Severe acute respiratory syndrome</term>
<term>Vaccine</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term>
<term>Prévention</term>
<term>Immunité humorale</term>
<term>Grave</term>
<term>Malade état grave</term>
<term>Aigu</term>
<term>Voie respiratoire</term>
<term>Conception</term>
<term>Protéine</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Vaccin</term>
<term>Immunoprophylaxie</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Forme grave</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Some of the structural proteins of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for the induction of protective humoral responses and the development of an effective vaccine. Rabbit antisera were prepared using full-length N and M proteins and eight expressed fragments covering the S protein. Antisera to S and M proteins were found to have different neutralizing titres towards SARS-CoV infection in vivo, ranging from 1:35 to 1:128. Antiserum to the N protein did not contain neutralizing antibodies. Epitopes inducing protective humoral responses to virus infection were located mainly in the M protein and a region spanning residues 13-877 of the S protein. The neutralizing ability of antisera directed against the expressed structural proteins was greater than that of convalescent patient antisera, confirming that, as immunogens, the former induce strong, SARS-CoV-specific neutralizing antibody responses. The in vitro neutralization assay has important implications for the design of an effective, protein-based vaccine preventing SARS-CoV infection.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Hai Pang" sort="Hai Pang" uniqKey="Hai Pang" last="Hai Pang">HAI PANG</name>
</noRegion>
<name sortKey="Bartlam, Mark" sort="Bartlam, Mark" uniqKey="Bartlam M" first="Mark" last="Bartlam">Mark Bartlam</name>
<name sortKey="Bartlam, Mark" sort="Bartlam, Mark" uniqKey="Bartlam M" first="Mark" last="Bartlam">Mark Bartlam</name>
<name sortKey="Donglai Wu" sort="Donglai Wu" uniqKey="Donglai Wu" last="Donglai Wu">DONGLAI WU</name>
<name sortKey="Fuguo Jiang" sort="Fuguo Jiang" uniqKey="Fuguo Jiang" last="Fuguo Jiang">FUGUO JIANG</name>
<name sortKey="Fuguo Jiang" sort="Fuguo Jiang" uniqKey="Fuguo Jiang" last="Fuguo Jiang">FUGUO JIANG</name>
<name sortKey="Hai Pang" sort="Hai Pang" uniqKey="Hai Pang" last="Hai Pang">HAI PANG</name>
<name sortKey="Xiangang Kong" sort="Xiangang Kong" uniqKey="Xiangang Kong" last="Xiangang Kong">XIANGANG KONG</name>
<name sortKey="Xueqing Han" sort="Xueqing Han" uniqKey="Xueqing Han" last="Xueqing Han">XUEQING HAN</name>
<name sortKey="Xueqing Han" sort="Xueqing Han" uniqKey="Xueqing Han" last="Xueqing Han">XUEQING HAN</name>
<name sortKey="Yanhui Xu" sort="Yanhui Xu" uniqKey="Yanhui Xu" last="Yanhui Xu">YANHUI XU</name>
<name sortKey="Yanhui Xu" sort="Yanhui Xu" uniqKey="Yanhui Xu" last="Yanhui Xu">YANHUI XU</name>
<name sortKey="Yinggang Liu" sort="Yinggang Liu" uniqKey="Yinggang Liu" last="Yinggang Liu">YINGGANG LIU</name>
<name sortKey="Zihe Rao" sort="Zihe Rao" uniqKey="Zihe Rao" last="Zihe Rao">ZIHE RAO</name>
<name sortKey="Zihe Rao" sort="Zihe Rao" uniqKey="Zihe Rao" last="Zihe Rao">ZIHE RAO</name>
</country>
</tree>
</affiliations>
</record>

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Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine

Protective humoral responses to severe acute respiratory syndrome-associated coronavirus: implications for the design of an effective protein-based vaccine

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